<p>The objective of the study was to validate the Maslach Burnout Inventory-Student Survey (MBI-SS) in university students of Physical Culture and Medicine from Holguín, Cuba. For this purpose, a cross-sectional validation study was carried out. Its content validity was determined by consulting experts (10), the degree of concordance of their answers with the Kendall concordance coefficient (W), and the reliability of the instrument through its internal consistency, calculating the Cronbach Alpha coefficient (C) of the instrument applied to 68 students of Holguin Physical Culture and Medicine. The results obtained show that the validity of the content is acceptable: W: 0.52 (Exhaustion: 0.64; Cynicism: 0.55; Effectiveness: 0.36). Its reliability is also acceptable, C: 0.70 (Exhaustion: 0.77; Cynicism: 0.59; Efficacy: 0.73). The MBI-SS is valid to be applied to the Cuban university student population, specifically to students of Holguín's physical culture and medicine. From now on, there will be an empirical diagnostic instrument that will allow us to determine the existence of this dangerous and harmful syndrome in our university population.</p>