<p>This study sought to assess psychological distress, life satisfaction, and perceived stress of Ecuadorian teachers who adopted online learning in response to the pandemic for COVID-19. A web-based cross-sectional survey was conducted, 394 teachers completed the questionnaire, 56.9% were females. Most of the participants (89.1%) were higher education teachers. Female teachers presented higher levels of perceived stress, and teachers with care responsibilities at home presented higher psychological distress and perceived stress. Teachers who had training for and experience with online teaching before the pandemic presented lower levels of distress and perceived stress, and higher levels of life satisfaction. Future studies must consider the understanding of work-family conflicts as well as social and structural inequalities that might have a toll on teachers’ mental health and performance.</p>