We investigated the process of constructing gender identity in a transgender girl through in-depth interviews with her parents, the girl and the application of graphic tests. The interviews were analyzed through the method of life stories, and then analyzed through content analysis. The results showed that the construction of trans gender identity is independent of the role of parents, but rather corresponds to processes of interaction between culture and the subject at an early age, where the transfer of gender meanings from the Culture is not passive, being that the boys and girls are active subjects in assimilating the genre that seems to them more attractive. Feminine gender meanings seem to be more striking and make them their own, accommodating the body according to the gendered meanings apprehended. It highlights the role of the school as a binary institution, which can be a triggering factor for depressive and anxious symptoms in transgender children, which refer to allowing these children to live according to their gender identity.