<p>Transition to telework can cause stress, loneliness and negative effects on the family life of teachers, thus affecting their quality of life. This study had as general objective to determine the quality of life of University of Cuenca’s professors during telework modality; and as specific objectives to describe the quality of life according to the physical, psychological, social and environmental domains; and to analyze the quality of life according to the sociodemographic characteristics of the professors. A quantitative, cross-sectional and exploratory research was carried out with a self-selected sample of 718 teachers. The World Health Organization Quality of Life Questionnaire - BREF (WHOQOL-BREF), Spanish version, was applied. The sociodemographic data were collected by means of a form prepared by the researcher in charge. The results obtained show that the University of Cuenca’s professors have a healthy quality of life profile that seems not to have been affected by the teleworking modality. In relation to the domains, the data indicate that during this modality the most affected domain is the social domain. Similarly, it is perceived that the quality of life is influenced by variables such as biological sex, age, cohabitation, presence of children under twelve years of age, hiring modality and professor’s faculty.</p>